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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">eifc</journal-id><journal-title-group><journal-title xml:lang="ru">Эпизоотология Иммунобиология Фармакология Санитария</journal-title><trans-title-group xml:lang="en"><trans-title>Epizootology Immunobiology Pharmacology Sanitation</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2224-168X</issn><publisher><publisher-name>Институт экспериментальной ветеринарии им. С.Н. Вышелесского</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">eifc-22</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ИММУНОБИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>IMMUNOBIOLOGY</subject></subj-group></article-categories><title-group><article-title>Выделение, культивирование и идентификация вируса деформации крыла пчелы медоносной (Apis mellifera L.) в Республике Беларусь</article-title><trans-title-group xml:lang="en"><trans-title>Isolation, cultivation and identification of deformed wing virus of honeybees (Apis mellifera L.) in the Republic of Belarus</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Черник</surname><given-names>М. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Chernik</surname><given-names>M. I.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Радюш</surname><given-names>И. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Radziush</surname><given-names>I. S.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Тяпша</surname><given-names>Ю. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Tyapsha</surname><given-names>Y. I.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Дубаневич</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Dubanevich</surname><given-names>O. V.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff xml:lang="ru" id="aff-1"><institution>РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»</institution><country>Russian Federation</country></aff><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>30</day><month>12</month><year>2018</year></pub-date><volume>0</volume><issue>2</issue><fpage>43</fpage><lpage>53</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Черник М.И., Радюш И.С., Тяпша Ю.И., Дубаневич О.В., 2018</copyright-statement><copyright-year>2018</copyright-year><copyright-holder xml:lang="ru">Черник М.И., Радюш И.С., Тяпша Ю.И., Дубаневич О.В.</copyright-holder><copyright-holder xml:lang="en">Chernik M.I., Radziush I.S., Tyapsha Y.I., Dubanevich O.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://eifc.belal.by/jour/article/view/22">https://eifc.belal.by/jour/article/view/22</self-uri><abstract><p>Сложность диагностики вирусных инфекций пчёл обусловлена отсутствием культур клеток тканей пчелы медоносной, коллекций штаммов вирусов, патогенных для медоносных пчел, и тест-систем для молекулярно-генетической идентификации. Из всех вирусов обнаруженных у медоносной пчелы Apis mellifera L., наибольшее распространение приобрел вирус деформации крыла. Впервые в Республике Беларусь сотрудниками РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского» выделен вирус деформации крыла из тканей пчелы медоносной с использованием первично-трипсинизированной культуры клеток фибробластов эмбрионов кур и проведена его молекулярно-генетическая идентификация.</p></abstract><trans-abstract xml:lang="en"><p>The complexity of diagnosing viral infections of bees is due to the lack of cultures of honeybee cells, collections of strains of viruses pathogenic for honeybees and test systems for molecular genetic identification. Of all the viruses found in the honeybee Apis mellifera L., the deformed wing virus has become the most common. For the first time in the Republic of Belarus, employees of the RUE ((Institute of Experimental Veterinary Medicine named S.N. Vyshelesky» isolated a deformed wing virus from the tissues of a honeybee using a primary trypsinized culture of chicken embryo fibroblast cells and carried out its molecular genetic identification.</p></trans-abstract></article-meta></front><back><ref-list><title>References</title></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
