Hyperimmunization of chickens egg breeds for obtaining yolk immunoglobulins specific to escherichia coli

This article presents the results of studies on the selection of adjuvants and the optimal scheme for hyperimmunization of Escherichia coli laying hens to obtain specific yolk immunoglobulins. It was found that to obtain specific yolk immunoglobulins, two immunizations of chickens with formalized Escherichia coli antigen (3x109 CFU/ml) with Montanide ISA 70 or Montanide ISA 206 adjuvant are sufficient. The use of Montanide ISA 70 in an adjuvant:antigen ratio of 70:30 by weight, respectively, and Montanide ISA 206 in an adjuvant:antigen ratio of 50:50 by weight, respectively, is equivalent.

1. Винокуров, В. Ю. Колибактериоз (эшерихиоз) кур: эпизоотология, диагностика, профилактика и меры борьбы : дис. … канд. ветеринар. наук : 06.02.02 / В. Ю. Винокуров. – Персиановский, 2010. – 120 л.

2. Каплин, В. С. Использование желточных антител птиц (IgY) для пассивной иммунизации сельскохозяйственных и домашних животных / В. С. Каплин, О. Н. Каплина // Ветеринария Кубани. – 2018. – № 4. – С. 19–24.

3. Каплин, В. С. IgY-технологии. Желточные антитела птиц / В. С. Каплин, О. Н. Каплина // Биотехнология. – 2017. – Т. 33, № 2. – С. 29–40.

4. Egg yolk antibodies (IgY) and their applications in human and veterinary health: а review / E.P.V. Pereira [et al.] // Int. Immunopharmacol. – 2019. – № 73. – P. 293–303.

5. Egg yolk IgY antibodies : a therapeutic intervention against group a rotavirus in calves / C. Vega [et al.] // Res. Vet. Sci. – 2015. – № 103. – P. 1–10.

6. Persister formation in Staphylococcus aureus is associated with ATP depletion / P. B. Conlon [еt al.] // Nature Microdiology. – 2016. – P. 1–7.

7. Production of Egg yolk immunoglobulin against Escherichia coli from White Leghorn and Lohmann chickens / J. F. Liou [et al.] // Journal of Animal and Veterinary Advances. – 2011. – Vol. 10, № 18. – P. 2349–2356.