Synthesis of antibody-antibiotic conjugates to combat bacterial infections
https://doi.org/10.47612/2224-168X-2022-1-31-36
Abstract
Design of antibody-antibiotic conjugates with doxycycline immunocompetent to Escherichia coli is proposed. The egg yolk immunoglobulins from hens immunized with Escherichia coli have been conjugated with the antibiotic using the glutaraldehyde as the crosslinking reagent. The reaction of conjugation was followed spectrophotometrically and revealed the formation of distinct absorption bands in ultraviolet regions. The kinetic analysis of forming the conjugates by the Guggenheim method showed that the conjugation reaction obeyed the kinetics of the first-order reactions. The velocity constant equaled 0,77 min-1. The half-conversion period of immunoglobulins during conjugation with glutaraldehyde was about 1 min. The reaction was rapid in aqueous solution at room temperature. The molar ratio of doxycycline: antibody in the conjugate was 2:1.
About the Authors
S. M. Yakubovsky
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
I. V. Nasonov
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
N. V. Knysh
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
I. S. Radyush
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
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Review
For citations:
Yakubovsky S.M., Nasonov I.V., Knysh N.V., Radyush I.S. Synthesis of antibody-antibiotic conjugates to combat bacterial infections. Epizootology Immunobiology Pharmacology Sanitation. 2022;(1):31-36. (In Russ.) https://doi.org/10.47612/2224-168X-2022-1-31-36
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