Development of fluorescence im- munochromatographic assay kit for african swine fever virus fast testing
Abstract
The continuing spread of African swine fever (ASF) outside Africa in the whole world, has heightened awareness of the threat posed by this devastating disease to the global pig industry and food security. Fast and accurate African swine fever virus (ASFV) detection method is very important for ASF prevention. A double antibody sandwich FICA method was optimized and established, and FICA strips (FICAS) were assembled and then evaluated. The sensitivity of the FICAS kit was 0,044 ng/mL. The recoveries ranged from 102,07 % to 108,60 %, and all CVs were below 7 %. The common pigs infectious disease samples, positive samples and healthy control samples did not lead to false negative and false positives. The kit can be stably stored at 37 °C for 7 days without significant decrease in fluorescence value. The prepared FICAS kit is rapid, feasible and effective for testing ASFV within 15 min. This study provides a new method for rapidly screening ASFV infection in pigs industry.
About the Authors
Laiqing Li
Guangzhou Youdi Bio-technology Co., Ltd.; Jinan Laide Bio-technology Co., Ltd.; Guangzhou Zhenda Biopharmaceutical Technology Co., Ltd.
China
China
Laiqing Li, chairman of the board
Guangzhou; Jinan
Cuicui Chen
Guangzhou Youdi Bio-technology Co., Ltd.; Jinan Laide Bio-technology Co., Ltd.
China
China
Cuicui Chen, project manager
Guangzhou; Jinan
Huankun Liang
Guangzhou Youdi Bio-technology Co., Ltd.
China
China
Huankun Liang, quality manager
Guangzhou
Tiancai Liu
Southern Medical University
China
China
Tiancai Liu
School of Laboratory Medicine and Biotechnology
Guangzhou
Wenqi Dong
Guangzhou Zhenda Biopharmaceutical Technology Co., Ltd.
China
China
Wenqi Dong
Guangzhou
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Review
For citations:
Li L., Chen C., Liang H., Liu T., Dong W. Development of fluorescence im- munochromatographic assay kit for african swine fever virus fast testing. Epizootology Immunobiology Pharmacology Sanitation. 2024;(1):39-44.
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