Obtaining producer strains of recombinant proteins of Mycoplasma hyopneumoniae and Mycoplasma bovis in the E. сoli expression system and evaluation of their antigenic properties
Abstract
The article describes production of recombinant strains-producers of Escherichia coli synthesizing recombinant proteins of M. bovis and M. hyopneumoniae and evaluation of antigenic properties of membrane proteins synthesized by them. Obtained results demonstrate expressed antigenic properties of recombinant proteins and confirm perspective of created strains-producers for obtaining standardised antigenic preparations, suitable for use in ELISA-test systems for diagnostics of farm animal mycoplasmosis.
About the Authors
Yu. I. Tyapsha
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
O. V. Dubanevich
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
References
1. Razin, S. Molecular biology and pathogenicity of mycoplasmas / S. Razin, D. Yogev, Y. Naot // Microbiol. Mol. Biol. Rev. – 1998. – Vol. 62, № 4. – P. 1094–1156.
2. Nicholas, R. A. J. Mycoplasma bovis: disease, diagnosis, and control / R. A. J. Nicholas, R. D. Ayling // Res. Vet. Sci. – 2003. – Vol. 74. – P. 105–112.
3. Review on the transmission of Mycoplasma hyopneumoniae in pig herds / D. Maes [et al.] // Vet. Res. – 2008. – Vol. 39, № 1. – Art. 18.
4. Проблемы лабораторной диагностики микоплазмозов животных / Р. Т. Сахарчук [и др.] // Ветеринарная патология. – 2018. – № 2. – С. 45–51.
5. Получение рекомбинантных антигенов для иммуноферментного анализа: стратегии и применение / О. В. Соколова [и др.] // Биотехнология. – 2019. – Т. 35, № 4. – С. 22–32.
6. Protective potential of recombinant non-purified botulinum neurotoxin serotypes C and D / Jr. C. Moreira [et al.] // Anaerobe. – 2016. – Vol. 40. – P. 58–62.
Review
For citations:
Tyapsha Yu.I., Dubanevich O.V. Obtaining producer strains of recombinant proteins of Mycoplasma hyopneumoniae and Mycoplasma bovis in the E. сoli expression system and evaluation of their antigenic properties. Epizootology Immunobiology Pharmacology Sanitation. 2025;(2):18-22. (In Russ.)
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