Development of ELISA-diagnosticum for detection of specific antibodies to African swine fever virus
Abstract
Recombinant antigens of the African swine fever virus (ASF) have been developed by cloning and expressing proteins encoded by two genes of this virus: p72 (B646L) and p30 (CP204L). The parameters of adsorption of recombinant proteins of the African swine fever virus have been worked out and optimal concentrations of ELISA diagnostic components have been determined for the detection of antibodies to antigens of the African swine fever virus. An ELISA diagnosticum has been developed to detect specific antibodies to the African swine fever virus. Rabbits were immunized and specific serums for the African swine fever virus were obtained. The selection of optimal concentrations of diagnostic components for the detection of antibodies to the ASF virus antigen was carried out. The developed ELISA diagnostic was tested in terms of specificity and sensitivity.
About the Authors
D. S. Borisovets
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
A. A. Rodriguez-Mallon
Центр генетической инженерии и биотехнологий
Cuba
Cuba
Yu. I. Tyapsha
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
A. A. Zgirovskaya
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
V. I. Gerasimenko
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
References
1. Кашкин, К. П. Методические разработки по клинической иммунологии / К. П. Кашкин, Е. Н. Степанова, Л. М. Скуинь. – М. : Медицина, 1997. – 518 с.
2. Gladue, D. P. Recombinant ASF live attenuated virus strains as experimental vaccine candidates / D. P. Gladue, M. V. Borca // Viruses. – 2022. – Vol. 14. – № 5. – P. 878.
3. Viral vector vaccines against ASF: Problems and prospectives / R. K. Ravilov [et al.] // Frontiers in Veterinary Science. – 2022. – Vol. 9. – P. 830244.
4. Vaccination with a gamma irradiation-inactivated African swine fever virus is safe but does not protect against a challenge / J. Pikalo [et al.] // Frontiers in Immunology. – 2022. – Vol. 13. – P. 832264.
5. Adenovirus-vectored African Swine Fever Virus antigen cocktails are immunogenic but not protective against intranasal challenge with Georgia 2007/1 isolate / S. Lokhandwala [et al.] // Veterinary microbiology. – 2019. – Vol. 235. – P. 10–20.
6. A solid-phase enzyme linked immunosorbent assay using monoclonal antibodies, for the detection of african swine fever virus antigens and antibodies / M. I. Vidal, M. Stiene, J. Henkel [et al.] // J. Virol. Methods. – 1997. – 66 (2). – Р. 211–218.
7. World Organization of Animal Health (OIE). African swine fever. Manual of Standards for diagnostic test and vaccines. – 2008.
8. High doses of inactivated African swine fever virus are safe, but do not confer protection against a virulent challenge / E. Cadenas-Fernández [et al.] // Vaccines. – 2021. – Vol. 9. – № 3. – P. 242.
9. Immunization of pigs by DNA prime and recombinant vaccinia virus boost to identify and rank African swine fever virus immunogenic and protective proteins / J. K. Jancovich [et al.] // Journal of virology. – 2018. – Vol. 92. – №. 8. – P. 10.1128/jvi.02219-17.
10. DNA-protein vaccination strategy does not protect from challenge with African swine fever virus Armenia 2007 strain / S. Y. Sunwoo [et al.] // Vaccines. – 2019. – Vol. 7, № 1. – P. 12.
11. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein-dye binding / M.M. Bradford // Anal. Biochem. – 1976. – Vol.72, № 1-2. – P. 248–254.
Review
For citations:
Borisovets D.S., Rodriguez-Mallon A.A., Tyapsha Yu.I., Zgirovskaya A.A., Gerasimenko V.I. Development of ELISA-diagnosticum for detection of specific antibodies to African swine fever virus. Epizootology Immunobiology Pharmacology Sanitation. 2025;(2):29-35. (In Russ.)
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