Development of ELISA-diagnosticum for detection of specific antibodies to African swine fever virus
Abstract
Recombinant antigens of the African swine fever virus (ASF) have been developed by cloning and expressing proteins encoded by two genes of this virus: p72 (B646L) and p30 (CP204L). The parameters of adsorption of recombinant proteins of the African swine fever virus have been worked out and optimal concentrations of ELISA diagnostic components have been determined for the detection of antibodies to antigens of the African swine fever virus. An ELISA diagnosticum has been developed to detect specific antibodies to the African swine fever virus. Rabbits were immunized and specific serums for the African swine fever virus were obtained. The selection of optimal concentrations of diagnostic components for the detection of antibodies to the ASF virus antigen was carried out. The developed ELISA diagnostic was tested in terms of specificity and sensitivity.
About the Authors
D. S. Borisovets
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
A. A. Rodriguez-Mallon
Центр генетической инженерии и биотехнологий
Cuba
Cuba
Yu. I. Tyapsha
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
A. A. Zgirovskaya
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
V. I. Gerasimenko
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
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Review
For citations:
Borisovets D.S., Rodriguez-Mallon A.A., Tyapsha Yu.I., Zgirovskaya A.A., Gerasimenko V.I. Development of ELISA-diagnosticum for detection of specific antibodies to African swine fever virus. Epizootology Immunobiology Pharmacology Sanitation. 2025;(2):29-35. (In Russ.)
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