Molecular genetic diagnosis of porcine reproductive and respiratory syndrome
Abstract
In the reproductive pathology of pigs, the porcine reproductive and respiratory syndrome virus (RRSS) plays a special role. For the rapid, highly sensitive and specific detection of the viral genome, molecular biological methods, primarily polymerase chain reaction (PCR), have become widespread. Modern developments are aimed at creating diagnostic test systems that allow high efficiency to identify the pathogen genome in the sample, which significantly reduces the time and cost of testing. The developed method makes it possible to identify the RRSS virus genome in various biological material (blood serum, parenchymal organs, virus isolates) with high sensitivity. The study of the above infection showed its widespread distribution in the pathology of pigs.
Thus, the seropositivity of pigs to the PRRS virus on individual pig farms is 77,5 %.
About the Authors
P. P. KrasochkoBelarus
D. S. Borisovets
Russian Federation
Bi Kaixuan
Russian Federation
References
1. Болезни вирусной этиологии репродуктивных органов свиноматок / П. А. Красочко, П. П. Красочко, Б. Кайсюань [и др.] // Наше сельское хозяйство. – 2021. – № 14 (262). – С. 52–56. – EDN JGGYPI.
2. Мальцева, Б. М. Смешанное течение репродуктивно-респираторного синдрома и парвовирусной инфекции свиней / Б. М. Мальцева // Ветеринария. Реферативный журнал. – 2001. – № 1. – С. 230.
3. Максимович, В. В. Инфекционные болезни свиней: монография – 2 изд., перераб. и доп. – Витебск : УО ВГАВМ, 2011. – 340 с. – EDN VYTLKJ.
4. WOAH Terrestrial Manual 2021: 3.9.6. Porcine reproductive and respiratory syndrome (infection with PRRS virus) - online version.
5. Co-infection dynamics of porcine parvovirus and PRRSV in European swine herds / I. Mészáros [et al.] // Veterinary Microbiology, 2019. – 231. – Р. 87–94.
6. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals: Chapter 3.9.6. Porcine reproductive and respiratory syndrome (infection with PRRS virus). – 2020. Online version.
7. A Novel Porcine Circovirus Distantly Related to Known Circoviruses Is Associated with Porcine Dermatitis and Nephropathy Syndrome and Reproductive Failure / R. Palinski, P. Piñeyro, P. Shang [et al.] // Journal of Virology. – 2016. – Vol. 91. – Is. 1. e01879-16. DOI 10.1128/JVI.01879-16.
Review
For citations:
Krasochko P.P., Borisovets D.S., Kaixuan B. Molecular genetic diagnosis of porcine reproductive and respiratory syndrome. Epizootology Immunobiology Pharmacology Sanitation. 2025;(2):36-40. (In Russ.)






