Ensitivity and specificity of multiplex PCR Pasteurella multocida A, B, D for detecting pasterella seroviariant

Abstract

Data on determination of specificity and sensitivity of multiplex PTsR of test system for diagnostics of Pasteurella multocida of serovariant of A, B, D are presented in article. Researches on studying of influence of nonspecific DNA on an amplifitsiruyemost of specific products are conducted, the mutually specificity of primers for serovariant of A, B, D and also formation of nespetsifichesy fragments without and in the presence of specific DNA is studied. The threshold of detectability of DNA for each Pasteurella multocida serovariant in a multiplex is established to system.