Mycobacteria of tuberculosis at lethal heat shock form protective forms which can pass through ultrafilters and restore viability as CWD mycobacteria

Abstract

Mycobacterium bovis 8 grew up on the Sauton medium, autoclaved (121 °C), filtered through Millex® GP 0.45, 0.22 µm and Amicon Ultracel® 100 K, 10K, 3K. Ultrafiltrates mixed with VKG growth stimulant, incubated and sowed on MycCel DW nutrient medium. It is established that mycobacteria of tuberculosis form the protective forms maintaining heating to 121 °C and pass through sterilizing (0.22 µm) and ultrafilters 10-3 nm, restoring viability as cell wall deficient forms. The nature of the established phenomens is not absolutely clear yet, but they should be considered at risk assessment of use of biological products, food and studying of pathologies not of a clear etiology.