Detection of the specific marker capC plasmides of pXO2 bacillus anthracis by means of PCR method in real time
Abstract
This paper represents qPCR validation results for the detection of Bacillus anthracis capC pXO2 plasmid marker. Amplification parameters as well as concentration of master mix components were optimized. Besides, we defined coefficients of variability within single and several independent experiments; analytical, diagnostical sensitivity and specificity. This validation procedure we conducted has proved robustness of this qPCR method, as well as allowed to improve its accuracy, repeatability and sensitivity.
About the Authors
A. V. Beloyvan
Национальный научный центр «Институт экспериментальной и клинической ветеринарной медицины»
Russian Federation
Russian Federation
B. T. Stegny
Национальный научный центр «Институт экспериментальной и клинической ветеринарной медицины»
Russian Federation
Russian Federation
A. P. Gerilovich
Национальный научный центр «Институт экспериментальной и клинической ветеринарной медицины»
Russian Federation
Russian Federation
A. S. Solodyankin
Национальный научный центр «Институт экспериментальной и клинической ветеринарной медицины»
Russian Federation
Russian Federation
A. .. Duerr
Институт микробиологии Бундесвера
Russian Federation
Russian Federation
J. .. Schwartz
Институт микробиологии Бундесвера
Russian Federation
Russian Federation
Review
For citations:
Beloyvan A.V., Stegny B.T., Gerilovich A.P., Solodyankin A.S., Duerr A..., Schwartz J... Detection of the specific marker capC plasmides of pXO2 bacillus anthracis by means of PCR method in real time. Epizootology Immunobiology Pharmacology Sanitation. 2019;(1):54-62. (In Russ.)
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