Determination of effective adjuvants and immunostimulators for hyperimmunization of chickens
D. S. Barysavets,
I. V. Nasonov,
T. A. Zuykevich,
P. A. Krasochko,
E. S. Zhurauleva,
N. V. Knysh,
N. V. Zinina,
A. A. Zgirouskaya,
Ya. P. Yaromchik,
D. V. Buchukuri,
S. M. Yakubousky,
A. E. Osipenko
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Abstract
The article presents data on the selection of adjuvants and immunostimulants was carried out to increase the production of transovarian immunoglobulins, the optimal scheme of hyperimmunization of laying hens was worked out. It was found that the most optimal adjuvant for hyperimmunization of laying hens is Montanide IMS 1313 VG, the use of which leds to the development of specific antibodies in titer of 5,67 log2. The use of Bacillus subtilis lipopolysaccharide as an immunostimulant leds to an increase in antiviral antibody titer at 0,17-0,83 log2. The optimal scheme for hyperimmunization of laying hens is use of antigens in the form of monocomponents at a dose of 0,5 cm3 intramuscularly into the pectoral muscle region four times with an interval of 10-14 days, which increases the level of specific antibodies at 1,0-1,67 log2.
About the Authors
D. S. Barysavets
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
I. V. Nasonov
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
T. A. Zuykevich
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
P. A. Krasochko
УО «Витебская ордена «Знак Почета» государственная академия ветеринарной медицины»
Belarus
Belarus
E. S. Zhurauleva
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
N. V. Knysh
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
N. V. Zinina
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
A. A. Zgirouskaya
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
Ya. P. Yaromchik
УО «Витебская ордена «Знак Почета» государственная академия ветеринарной медицины»
Belarus
Belarus
D. V. Buchukuri
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
S. M. Yakubousky
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
A. E. Osipenko
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
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Review
For citations:
Barysavets D.S., Nasonov I.V., Zuykevich T.A., Krasochko P.A., Zhurauleva E.S., Knysh N.V., Zinina N.V., Zgirouskaya A.A., Yaromchik Ya.P., Buchukuri D.V., Yakubousky S.M., Osipenko A.E. Determination of effective adjuvants and immunostimulators for hyperimmunization of chickens. Epizootology Immunobiology Pharmacology Sanitation. 2020;(2):43-47. (In Russ.)
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