Determination of effective adjuvants and immunostimulators for hyperimmunization of chickens
Abstract
The article presents data on the selection of adjuvants and immunostimulants was carried out to increase the production of transovarian immunoglobulins, the optimal scheme of hyperimmunization of laying hens was worked out. It was found that the most optimal adjuvant for hyperimmunization of laying hens is Montanide IMS 1313 VG, the use of which leds to the development of specific antibodies in titer of 5,67 log2. The use of Bacillus subtilis lipopolysaccharide as an immunostimulant leds to an increase in antiviral antibody titer at 0,17-0,83 log2. The optimal scheme for hyperimmunization of laying hens is use of antigens in the form of monocomponents at a dose of 0,5 cm3 intramuscularly into the pectoral muscle region four times with an interval of 10-14 days, which increases the level of specific antibodies at 1,0-1,67 log2.
About the Authors
D. S. Barysavets
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
I. V. Nasonov
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
T. A. Zuykevich
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
P. A. Krasochko
УО «Витебская ордена «Знак Почета» государственная академия ветеринарной медицины»
Belarus
Belarus
E. S. Zhurauleva
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
N. V. Knysh
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
N. V. Zinina
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
A. A. Zgirouskaya
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
Ya. P. Yaromchik
УО «Витебская ордена «Знак Почета» государственная академия ветеринарной медицины»
Belarus
Belarus
D. V. Buchukuri
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
S. M. Yakubousky
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
A. E. Osipenko
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
References
1. Каплин, В. С. IgY-технологии в медицине. Желточные антитела птиц в иммунотерапии / В. С. Каплин, О. Н. Каплина // Международные обзоры: клиническая практика и здоровье. – 2016. – № 4. – С. 59–75.
2. Akita, E. M. Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain / E. M. Akita, S. Nakai // Journal of immunological methods. – 1993. – Vol. 160. – № 2. – P. 207–214.
3. De Meulenaer, B. Isolation and purification of chicken egg yolk immunoglobulins: a review / B. De Meulenaer, A. Huyghebaert // Food and Agricultural Immunology. – 2001. – Vol. 13. – № 4. – P. 275–288.
4. Development and immunochemical evaluation of antibodies Y for the poorly immunogenic polypeptide prothymosin alpha / P. Klimentzou [et al.] // Peptides. – 2006. – Vol. 27. – № 1. – P. 183–193.
5. Monitoring of laying capacity, immunoglobulin Y concentration, and antibody titer development in chickens immunized with ricin and botulinum toxins over a two-year period / D. Pauly [et al.] // Poultry Science. – 2009. – Vol. 88. – № 2. – P. 281–290.
6. Polson, A. Isolation of viral IgY antibodies from yolks of immunized hens / A. Polson, M. B. von Wechmar, M. H. V. Van Regenmortel // Immunological communications. – 1980. – Vol. 9. – № 5. – P. 475–493.
7. Chicken egg yolk antibodies, production and application: IgY-Technology / R. Schade [et al.] (ed.). – Springer Science & Business Media, 2000.
8. Chicken egg yolk antibodies (IgY-technology): a review of progress in production and use in research and human and veterinary medicine / R. Schade [et al.] // Alternatives to laboratory animals. – 2005. – Vol. 33. – № 2. – P. 129–154.
9. The crystal structure of an avian IgY-Fc fragment reveals conservation with both mammalian IgG and IgE / A. I. Taylor [et al.] // Biochemistry. – 2009. – Vol. 48. – № 3. – P. 558–562.
10. Gene gun-supported DNA immunisation of chicken for straightforward production of poxvirus-specific IgY antibodies / P. T. Witkowski [et al.] // Journal of immunological methods. – 2009. – Vol. 341. – № 1–2. – P. 146–153.
Review
For citations:
Barysavets D.S., Nasonov I.V., Zuykevich T.A., Krasochko P.A., Zhurauleva E.S., Knysh N.V., Zinina N.V., Zgirouskaya A.A., Yaromchik Ya.P., Buchukuri D.V., Yakubousky S.M., Osipenko A.E. Determination of effective adjuvants and immunostimulators for hyperimmunization of chickens. Epizootology Immunobiology Pharmacology Sanitation. 2020;(2):43-47. (In Russ.)
Views: 207
Email this article 