Determination of the optimal composition of antigens for obtaining of yolk immunoglobulins in hyperimmunization of nesting chickens
Abstract
The article provides data on the development of a method for obtaining transovarian immunoglobulins, one of the stages of which was to determine the optimal composition and ratio of antigens of viruses and bacteria for hyperimmunization of laying hens. As a result of the studies, it was found that in modern conditions of livestock farming with, the most common pathogens of infectious enteritis of calves are bovine viral diarrhea virus, bovine herpesvirus-1, rotavirus and coronavirus, as well as Escherichia coli bacteria, the production strains of which were used to immunize laying hens in the form of monocomponents. Hyperimmunization of poultry according to the developed scheme leds to the development of specific antibodies in titers – 5,0–8,0 log2, which is 2,0–5,0 log2 higher in comparison with the combined administration of antigens.
About the Authors
D. S. BarysavetsBelarus
I. V. Nasonov
Belarus
T. A. Zuykevich
Belarus
P.. A Krasochko
Belarus
E. S. Zhurauleva
Belarus
N. V. Knysh
Belarus
N. V. Zinina
Belarus
Ya. P. Yaromchik
Belarus
D. V. Buchukuri
Belarus
S. M. Yakubousky
Belarus
A. E. Osipenko
Belarus
References
1. Анализ заболеваемости молодняка КРС респираторными инфекциями / В. А. Мищенко [и др.] // Ветеринария Кубани. – 2008. – № 6. – С. 2–4.
2. Диагностика пневмоэнтеритов молодняка крупного рогатого скота в Республике Беларусь / А. Н. Притыченко [и др.] // Ученые записки учреждения образования «Витебская ордена «Знак почета» государственная академия ветеринарной медицины». – 2012. – Т. 48, № 1. – С. 54–59.
3. Каплин, В. С. IgY-технологии в медицине. Желточные антитела птиц в иммунотерапии / В. С. Каплин, О. Н. Каплина // Международные обзоры: клиническая практика и здоровье. – 2016. – № 4. – С. 59–75.
4. Руководство по вакцинному и сывороточному делу. – М., 1978. – 440 с. Руководство по профилактике в практическом здравоохранении / под ред. И. С. Глазунова, Р. Г. Оганова [и др.] – М., 2000. – 217 с.
5. Mine, Y. Chicken egg yolk antibodies as therapeutics in enteric infectious disease: a review / Y. Mine, J. Kovacs-Nolan // Journal of medicinal food. – 2002. – Vol. 5. – № 3. – P. 159–169.
6. Good effect of IgY against Pseudomonas aeruginosa infections in cystic fibrosis patients / E. Nilsson [et al.] // Pediatric pulmonology. – 2008. – Vol. 43. – № 9. – P. 892–899.
7. Avian IgY antibodies and their recombinant equivalents in research, diagnostics and therapy / E. Spillner [et al.] // Biologicals. – 2012. – Vol. 40. – № 5. – P. 313–322.
Review
For citations:
Barysavets D.S., Nasonov I.V., Zuykevich T.A., Krasochko P.A., Zhurauleva E.S., Knysh N.V., Zinina N.V., Yaromchik Ya.P., Buchukuri D.V., Yakubousky S.M., Osipenko A.E. Determination of the optimal composition of antigens for obtaining of yolk immunoglobulins in hyperimmunization of nesting chickens. Epizootology Immunobiology Pharmacology Sanitation. 2020;(2):63-68. (In Russ.)