The article presents modern literature data on the susceptibility of different animal species, incl. minks to the coronavirus infection caused by the SARS-CoV-2 virus

The article presents data on the pathoanatomical picture of the internal organs of pigs of different ages, and studies various biological fluids from pigs of different ages with the aim of using them in the diagnosis of pig circovirus infection. It has been established that the most accessible and informative biological fluid is nasal mucus and sperm. The genome of circovirus type 2 is also isolated from feces.

Isolation of the circovirus genome from pathological material indicates that the largest amount of the genome equivalent is isolated from pigs at the age of 90 days.

The effectiveness of using serum produced by the RUP «Institute of Experimental Veterinary Science of S.N. Vyshelessky», obtained from cattle, for culturing the transplanted cell line CV-1 for the purpose of producing vaccines and veterinary immunobiological preparations, was studied. The received results of researches demonstrate that for the intertwined cellular CV-1 line the use of serum of blood of cattle produced by the RUP «Institute of Experimental Veterinary Science of S.N. Vyshelessky» on the immunobiological properties and quality characteristics doesn't concede to embryonic serum of calfs of production SIGMA.

The purpose of the research is to study the therapeutic and prophylactic effectiveness of a complex drug based on nano- and colloidal silver particles «Nanoargovir» for the prevention and treatment of respiratory infections in calves. It was established that this preparation has 85-91.7% preventive and 88.4-92% therapeutic effectiveness for calves.

Cell cryopreservation has been shown to be a promising method in scientific research. It is important to comply with all conditions for the process of thawing cell cultures for their use in the manufacture of immunobiological preparations. It has been established that the presence of characteristic signs of cells spreading onto the substrate during the first three days after seeding indicates the completion of the process of proliferation of cells preserved after cryopreservation. Optimal results of restoring a nuclear cell culture in a freeze-thaw cycle were achieved using Eagle growth medium: DMEM with the addition of 10 % cattle blood serum and a cell concentration of 2,2×106. Under these conditions and the freezing method we chose, the studied culture of NDC cells was completely restored by the 5th day of cultivation. The tested cell culture, in the process of thawing as the duration of cultivation increased, did not undergo genetic changes, had a fibroblast-like morphology characteristic of this line, and retained its biological properties.

An analysis of literary sources available in the open press showed that genetic engineering is one of the most promising areas in the field of creating a number of antiviral drugs for veterinary medicine. The use of genetic engineering technologies in the development of effective means of controlling animal viral infections is a relevant area for the scientific community, as it will prevent the development of diseases in livestock farms and preserve the health of the herd.

The MTT assay for cellular metabolic activity is widely used in vitro. This paper presents our own data and critical assessment of the literature data is carried out. The applicability and potential limitations of the MTT assay are discussed.

The compositions of theotropin and quaternary ammonium compounds had a pronounced effect on epizootically significant species of bacteria and fungi at a concentration of theotropin of 0,2–0,5 % (depending on the species), and the effectiveness of the action was not reduced by organic protection. The composition of theotropin, quaternary ammonium compound and surfactant in 2 % concentration with short-term contact (up to 30 min) bacteriostatically acted on Mycobacterium bovis, and in 3 % concentration ensured their inactivation on surfaces with organic protection 88,8 % of the population.

The tendency of microflora resistance to 1 % theotropin-based compositions increased more slowly than to a chlorine-containing disinfectant, but this effect was manifested to a lesser extent when using 0,5 % working solution.

The optimal variant of the disinfectant composition based on theotropin can be used as a new disinfectant for veterinary surveillance facilities.

The article provides an overview of mycotoxin contamination of animal feed. The article includes information on the modern laboratory methods to identify and assess the degree of various mycotoxins’ contamination. The most promising adsorbents of the group of the biological methods used to reduce the degree of mycotoxins in animal feed are presented.